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PIP5K3 (PIKFYVE), Active
产品性质
| recombinant【重组】 | expressed in baculovirus infected Sf9 cells |
| Assay【测定】 | 70-95% (SDS-PAGE) |
| form【形式】 | frozen liquid |
| Specific Activity | 90-115 nmol/min·mg (ADP-Glo) |
| mol wt【分子量】 | ~110 kDa |
| concentration【浓度】 | 0.05-0.1 μg/μL |
| accession no.【登记号】 | NM_015040 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −70℃ |
| Gene Information | human ... PIP5K3(200576) |
基本信息
| Application【应用】 | Useful for kinase assay. |
| Biochem/physiol Actions【生化/生理作用】 | Recombinant human PIP5K3 (1400-end) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. PIP5K3 (also known as phosphatidylinositol-3-phosphate 5-kinase type III or PIPKIII) encodes an enzyme that phosphorylates the D-5 position in PtdIns and phospha-tidylinositol-3-phosphate (PtdIns3P) to make PtdIns5P and PtdIns (3,5)biphosphate. PIP5K3 belongs to a large family of lipid kinases that alter the phosphorylation status of intracellular phosphatidylinositol but also have protein kinase activity (1). PIP5K3 regulate cytoskeletal functions, membrane trafficking, and receptor signaling by recruiting protein complexes to cell- and endosomal-membranes. The inhibition of PIP5K3 renders the late endosome /lysosome compartment refractory to fusion with autop-hagosomes and multivesicular bodies (2). |
| Recommended products【推荐产品】 | Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol. |
| Legal Information【法律信息】 | PRECISIO is a registered trademark of sigma-aldrich Co. LLC |
产品说明
| Storage and Stability【储存及稳定性】 | Store in aliquots at -70℃. Avoid freeze/thaw cycles. |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 1 |
