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T4 Beta-glucosyltransferase
| 产品编号： | 4140841 |
| 规格： | recombinant, expressed in E. coli, ≥83% (SDS-PAGE) |
| 包装规格： | 100 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
T4 Beta-glucosyltransferase
hydroxymethyl cytosine
UDP glucose-DNA ?-glucosyltransferase
T4-phage beta-glucosyltransferase
基本信息
| NACRES | NA.32 |
| General description【一般描述】 | T4-phage β−glucosyltransferase, also known as UDP glucose-DNA β-glucosyltransferase, (Genbank Accession No. NP_049658) amino acids 1-351(end) with C-terminal His-tag, MW= 41.6 kDa, expressed in Escherichia coli. |
| Application【应用】 | Useful for the differentiation of hydroxymethylcytosine (HMC) from methylcytosine in DNA, via glucosylating HMC and protecting HMC from endonuclease cleavage. |
| Biochem/physiol Actions【生化/生理作用】 | T4-phage β-glucosyltransferase is involved in the transfer of glucose from uridine diphosphoglucose (UDP-Glc) to 5-hydroxymethylcytosine (5-HMC) in double-stranded DNA of the T4-phage. Ions such as Mg2+, Mn2+ and Ca2+ activate this enzyme. |
| Physical form【外形】 | Formulated in 200 mM imidazole and 20% glycerol. |
产品性质
| biological source【生物来源】 | Escherichia coli |
| recombinant【重组】 | expressed in E. coli |
| Assay【测定】 | ≥83% (SDS-PAGE) |
| form【形式】 | aqueous solution |
| mol wt【分子量】 | 41.6 kDa |
| packaging【包装】 | pkg of 100 μg |
| NCBI accession no.【NCBI登记号】 | NP_049658 |
| UniProt accession no.【UniProt登记号】 | P04547 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −70℃ |
| Gene Information | bacteriophage T4 ... B-GT(1258765) |
安全信息
| Pictograms【象形图】 | GHS07,GHS08 |
| Signal word【警示用语：】 | Danger |
| Hazard Statements | H315 - H319 - H360D |
| Precautionary Statements | P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313 |
| Hazard Classifications【危险分类】 | Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2 |
| Storage Class Code【储存分类代码】 | 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects |
| WGK | WGK 1 |
