TRIS醋酸盐-EDTA缓冲液

MFCD00236357

NA.21

TAE 电泳缓冲液是 DNA 琼脂糖凝胶电泳最常用的缓冲液，还用于非变性 RNA 琼脂糖凝胶电泳。双链 DNA 在 TAE 中往往比在其他缓冲液中移动得更快，但在长时电泳期间也会因缓冲液离子耗尽而无法泳动。在长时电泳期间进行缓冲液循环或缓冲液更换可弥补较低的缓冲能力。稀释浓缩的 TAE 缓冲液得到 1× TAE 缓冲液，含有 40mM Tris 乙酸盐和 1mM EDTA，pH 为 8.3。1× TAE 缓冲液既可用于琼脂糖凝胶中，也可用作电泳缓冲液。为达到最大分辨率，建议外加电压低于 5V/cm(单位电极间的距离)。

采用经生物技术性能认证的 Trizma 碱 (T6066) 和分子生物学试剂 EDTA (E5134) 制备。溶液还含有乙酸 (A6283)；粉末状混合物含有 Trizma 醋酸盐 (T1258)。

Contents of one pouch, when dissolved in 500mL of distilled or deionized water, will yield a 1X solution containing 40mM Tris-Acetate, 1mM EDTA, pH 8.3 at 25 ℃. Certified to be DNAse, RNAse, Protease, and Nickase free. Certified for use in electrophoresis.

pHast Pack is a trademark of sigma-aldrich Co. LLC

pHast Pack^™

powder

8.3

suitable for gel electrophoresis (after dilution to working concentration)

CC(O)=O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

1S/C10H16N2O8.C4H11NO3.C2H4O2/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;1-2(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;1H3,(H,3,4)

HGEVZDLYZYVYHD-UHFFFAOYSA-N

Foil pouches

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

11 - Combustible Solids

WGK 3