Anti-CD18 Mouse mAb (IB4)

100

mouse

purified antibody

primary antibodies

IB4, monoclonal

≥95% (SDS-PAGE)

liquid

0.5 mg/mL gentamicin sulfate as preservative

human

Calbiochem^®

OK to freeze

IgG2a

wet ice

2-8℃

100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

Anti-CD18, mouse monoclonal, clone IB4, recognizes the ~95 kDa β2 integrin subunit. It is validated for FC, IP, IHC on frozen sections, and cell inhibition assays.
Protein A purified mouse monoclonal antibody. Recognizes the ~95 kDa β2 integrin subunit (CD18).
Recognizes the ~95 kDa β2 integrin subunit. Blocks the binding of ICAM-1 and ICAM-3 to LFA-1.

Human
human peripheral blood monocytes

Flow Cytometry (5-10 µg/ml)

Frozen Sections (see comments)

Immunoprecipitation (see comments)

Cell Inhibition Assays (see comments)

Toxicity: Standard Handling (A)

In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.

Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.

Negative Control
Nalm-6 cells
Positive Control
HPB-MLT cells

Berman, M.E., et al. 1996. J. Immunol.156, 1515.
Kaneko, M., et al. 1995. J. Immunol. 155, 2631.
Mazzone, A., and Ricevuti, G. 1995. Haematologica80, 161.
Kaneko, M., et al. 1995. J. Immunol.155, 2631.
Landis, R.C., et al. 1994. J. Cell Biol.126, 529.
Wright, S.D., et al. 1983. Proc. Natl. Acad. Sci. USA80, 5699.
Blocks the binding of ICAM-1 and ICAM-3 to LFA-1. FACS was performed on 5x10⁵ cultured human HPB-MLT cells using anti-CD18 antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody is also reported to work for immunoprecipitation and frozen sections. Variables associated with assay conditions will dictate the proper working dilution.

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10 - Combustible liquids

WGK 1