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Anti-Caspase-8 Antibody, clone 3B10
产品别名
Anti-Caspase-8 Antibody, clone 3B10
EC: 3.4.22.61, CASP-8, MCH5, FLICE, MACH
基本信息
| eCl@ss | 32160702 |
| General description【一般描述】 | Caspase-8 (UniProt: O89110; also known as EC: 3.4.22.61, CASP-8, CASP8, MCH5, FLICE, MACH) is encoded by the Casp8 gene (Gene ID: 12370) in murine species. Caspase-8 is a cysteine protease that plays a major role in the extrinsic apoptotic signaling pathway via death receptors. In its inactive form it associates with FADD by interactions between their DED motifs and within the FAS-FADD-Caspase 8 complex caspase 8 undergoes self-cleavage to convert to the active form. Activated caspase 8 is subsequently released to the cytosol and it cleaves downstream effector caspases 3, 4, 6, 7, 9 and 10. Caspase 8 is expressed in wide variety of tissues and its highest expression has been reported in spleen, thymus, lung, liver, and kidney. Low levels of expression are observed in heart, brain, testis, and skeletal muscle. Caspase-8 is produced with propetides (aa 1-218 and 377-387) that are cleaved under appropriate signals to produce a p20 (aa 219-376) and a p10 (aa 388-480) subunit. Active caspase 8 is a heterotetramer that consists of two p20 and two p10 subunits. |
| Specificity【特异性】 | This rat monoclonal antibody detects Caspase-8 in murine species. It targets an epitope within the p20 subunit. |
| Immunogen【免疫原】 | Recombinant p20 subunit of Mouse Caspase-8. |
| Application【应用】 | Flow Cytometry Analysis: 1 µg from a representative lot detected Caspase-8 in one million mouse embryonic fibroblasts (MEF-1). Anti-Caspase-8, clone 3B10, Cat. No. MABC1606, is a rat monoclonal antibody that detects Caspase-8 and has been tested for use in Flow Cytometry and Western Blotting. Research Category Apoptosis & Cancer |
| Quality【质量】 | Evaluated by Western Blotting in mouse embryonic fibroblast (MEF-1) cell lysates. Western Blotting Analysis: 1 µg/mL of this antibody detected Caspase-8 in mouse embryonic fibroblast (MEF-1) cell lysates. |
| Physical form【外形】 | Protein G purified Format: Purified Purified rat monoclonal antibody IgG1 in PBS with 0.02% sodium azide. |
| Other Notes【其他说明】 | Concentration: Please refer to lot specific datasheet. |
产品性质
| biological source【生物来源】 | rat |
| antibody form【抗体形式】 | purified antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | 3B10, monoclonal |
| species reactivity | mouse |
| packaging【包装】 | antibody small pack of 25 μg |
| technique(s) | flow cytometry: suitable western blot: suitable |
| isotype【同位素/亚型】 | IgG1κ |
| NCBI accession no.【NCBI登记号】 | NP_001073595 |
| UniProt accession no.【UniProt登记号】 | O89110 |
产品说明
| Target description【目标描述】 | ~55 kDa observed; 55.36 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
