Anti-CD44 Rat mAb (A020)

100

rat

purified antibody

primary antibodies

A020, monoclonal

liquid

≤0.1% sodium azide as preservative

rabbit, canine, mouse, human, bovine, porcine

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles

IgG2b

wet ice

−20℃

100 μL in Plastic ampoule
Please refer to vial label for lot-specific concentration.

Rat monoclonal antibody purified by ammonium sulfate precipitation and DEAE chromatography. Recognizes the ~85-95 kDa CD44 protein.
Recognizes the ~85-95 kDa CD44 protein (gp90) in human and GP85 or Pgp-1 in mouse.
Anti-CD44, rat monoclonal, clone A020, recognizes the ~85-95 kDa CD44 protein (gp90) in human and GP85 in mouse. It is validated for ELISA, IF, IP, RIA, WB, and IHC.

purified human lymphocyte CD44
Human

ELISA (1:2000)

Flow Cytometry (1:1000)

Immunoblotting (1:1000)

Immunofluorescence (1:200)

Immunohistochemistry (1:250)

Immunoprecipitation (1:200)

Neutralization Studies (see comments and application references)

Radioimmunoassay (1:5000)

Toxicity: Standard Handling (A)

In 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.

Following initial thaw, aliquot and freeze (-20°C).

The antibody specifically recognizes and reacts with a common determinant of the CD44 class of glycoproteins. This antibody has been reported to block the interaction between hyaluronan and CD44 (see application references). Variables associated with assay conditions will dictate the proper working dilution.
Iida, N., and Bourguignon, L.Y.W. 1995. J. Cell Physiol.162, 121.
Bourguignon, L.Y.W., et al. 1993. J. Immunol.151, 6634.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

12 - Non Combustible Liquids

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