Anti-MBP (Citrulline R122) Antibody, clone 1H1

32160702

Myelin basic protein (UniProt: P02686; also known as MBP, Myelin A1 protein, Myelin membrane encephalitogenic protein) is encoded by the MBP gene (Gene ID: 4155) in human. MBP is a homodimeric protein that is found in both the central and the peripheral nervous system. It is one of the most abundant protein component of the myelin membrane in the CNS and plays a role in both the formation and stabilization of this myelin membrane. It is responsible for adhesion of the cytosolic surfaces of multilayered compact myelin. MBP can interact with many polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and with negatively charged lipids. MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system. MBP expression begins abruptly in 14-16-week-old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. MBP citrullination by peptidylarginine deiminase (PAD) leads to incomplete protein-lipid bilayer interactions and increases its vulnerability to proteolytic enzymes, resulting in disorganization of the myelin sheath in the central nervous system. Citrullinated MBP (citMBP) has been suggested to be a hallmark of demyelination. MBP citrullination has frequently been demonstrated in various CNS disorders. citMBP at R25, R122, and R130 is reported in the brains of subjects with Creutzfeldt-Jakob disease (CJD). Clone 1H1 specifically recognizes citMBP at R122. (Ref.: Jang, B., et al. (2017). Mol. Neurobiol. 55(4); 3172-3184; Boggs, JM (2006). Cell. Mol. Life Sci. 63(17); 1945-1961).

Clone 1H1 is a mouse monoclonal antibody that detects human and murine myelin basic protein citrullinated on residue 122. It targets an epitope with in 19 amino acids from the C-terminal half.

KLH-conjugated linear peptide corresponding to 19 amino acids from isoform 5 of human Myelin Basic Protein with citrullination on residue 122.

Research Category
Cell Structure
Anti-MBP (Citrulline R122), clone 1H1, Cat. No. MABT1499, is a mouse monoclonal antibody that detects Myelin basic protein citrullinated on residue 122 and has been is tested for use in ELISA, Immunohistochemistry, and Western Blotting.
ELISA Analysis: A representative lot detected MBP (Citrulline R122) in ELISA applications (Jang, B., et. al. (2018). Mol Neurobiol. 55(4):3172-3184).

Western Blotting Analysis: A representative lot detected MBP (Citrulline R122) in Western Blotting applications (Jang, B., et. al. (2018). Mol Neurobiol. 55(4):3172-3184).

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MBP (Citrulline R122) in Myelin basic protein (MBP) treated with human PAD2 (Courtesy of Byungki Jang, Ph.D./Eun-Kyoung Choi, Ph.D., Ilsong Institute of Life Science, Hallym University, South Korea).





Immunohistochemistry Analysis: A representative lot detected MBP (Citrulline R122) in Immunohistochemistry applications (Jang, B., et. al. (2018). Mol Neurobiol. 55(4):3172-3184).

(Courtesy of Byungki Jang, Ph.D./Eun-Kyoung Choi, Ph.D., Industry Academic Cooperation Foundation, Hallym University, South Korea).

Evaluated by Western Blotting in GST-tagged myelin basic protein (MBP) treated with human PAD-2.

Western Blotting Analysis: 1 µg/mL of this antibody detected MBP (Citrulline R122) in GST-tagged myelin basic protein (MBP) treated with human Peptidyl Arginine Deiminase 2 (PAD-2)(200 ng for 4 h at 37°C).

Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Concentration: Please refer to lot specific datasheet.

mouse

purified immunoglobulin

primary antibodies

1H1, monoclonal

mouse, human

antibody small pack of 25 μg

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

IgG1κ

NP_001020252

P02686

~41 kDa observed; 18.59 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Stable for 1 year at 2-8°C from date of receipt.

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