PhosphoDetect Anti-Phosphotyrosine Mouse mAb (PY20) Peroxidase Conjugate

100

mouse

purified antibody

primary antibodies

PY20, monoclonal

liquid

0.1% Proclin as preservative

all

Calbiochem^®

OK to freeze

IgG2b

wet ice

2-8℃

100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

Purified mouse monoclonal antibody conjugated to horseradish peroxidase. Recognizes a broad spectrum of tyrosine-phosphorylated proteins.
Peroxidase conjugate of Anti-Phosphotyrosine Mouse mAb (PY20), Cat. No. 525295. Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states in crude extracts.
This PhosphoDetect Anti-Phosphotyrosine Mouse mAb (PY20) Peroxidase Conjugate is validated for use in ELISA, Immunoblotting, Immunohistochemistry for the detection of Phosphotyrosine.

KLH conjugated to phosphotyrosine, glycine, and alanine

ELISA (1:2000)

Immunoblotting (1:500-1:2000)

Immunohistochemistry (1:500)

Toxicity: Standard Handling (A)

In PBS, 50% glycerol, 1% BSA, pH 7.4.

Glenney, J.R., et al. 1988. J. Immunol. Methods109, 277.
Specific for tyrosine phosphorylated proteins in both the native and denatured states. Binding is inhibited by phosphotyrosine; does not react with threonine or serine phosphorylated residues on proteins. Do not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosine containing proteins and will adsorb the antibody. Use a buffer with <150 mM NaCl and divalent cations not much >1 mM because this antibody′s binding is inhibited by high salt concentrations and divalent cations. Variables associated with assay conditions will dictate the proper working dilution.

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10 - Combustible liquids

WGK 1