PhosphoDetect Anti-RhoA (pSer¹⁸⁸) Rabbit pAb

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

liquid

≤0.1% sodium azide as preservative

human

Calbiochem^®

OK to freeze

IgG

wet ice

−20℃

100 μL in Plastic ampoule
Please refer to vial label for lot-specific concentration.

This PhosphoDetect Anti-RhoA (pSer¹⁸⁸) Rabbit pAb is validated for use in Immunoblotting, ELISA for the detection of RhoA (pSer¹⁸⁸).
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~22 kDa RhoA protein when expressed as a GST fusion protein (total ~49 kDa) and phosphorylated in vitro by PKA.
Recognizes the ~22 kDa RhoA protein, expressed as a GST fusion protein (total ~49 kDa) and phosphorylated in vitro by PKA.

a synthetic peptide corresponding to amino acids surrounding the Ser¹⁸⁸ phosphorylation site of human RhoA, coupled to KLH
Human

Immunoblotting (1:1000)
ELISA (1:2000)

Toxicity: Standard Handling (A)

In PBS, 1 mg/ml BSA, 50% glycerol.

Do not aliquot.

Positive Control
RhoA GST fusion protein

Ellerbroek, S.M., et al. 2003. J. Biol. Chem.278, 19023.
Sawada, N., et al. 2001. Biochem. Biophys. Res. Commun. 280, 798.
Dong, J.M., et al. 1998. J. Biol. Chem.273, 22554.
Lang, P., et al. 1996. EMBO J.15, 510.
Does not recognize unphosphorylated RhoA. The immunogen sequence is conserved in rat and mouse RhoA, but cross-reactivity has not been tested. The immunogen sequence is not found in the RhoB and RhoC sequences. For immunoblotting, the membrane was incubated with antibody diluted in PBS/5% nonfat milk/0.04% Tween^® 20 detergent for 1 h at room temperature. Antibody should be titrated for optimal results in individual systems.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

10 - Combustible liquids

WGK 1