Sphingolipid Ceramide N-Deacylase, Pseudomonas sp.

bacterial (Pseudomonas spp.)

100

liquid

≥1 units/mL

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles

α- and β-N-acetylgalactosaminidase, α-fucosidase, α- and β-galactosidase, neuraminidases, none detected

wet ice

−20℃

250 miu in Plastic ampoule

Sphingolipid ceramide N-deacylase (SCDase) is a native sphingolipid ceramide N-deacylase from Pseudomonas species. It hydrolyzes the N-acyl linkage between fatty acids and the sphingosine base of ceramide in several sphingolipids and gangliosides. This enzyme catalyzes the condensation reaction between fatty acids and sphingosine bases and forms sphingolipids. It is also involved in transacylation reactions. SCDase is not recommended for hydrolysis of ceramide. It is also used to prepare lysoglycosphingolipids (lysoGSLs) effortlessly.

Sphingolipid ceramide N-Deacylase, Pseudomonas sp. has been used as a PP2A enhancer to study its effects on phosphatase and tensin homolog (PTEN) in forkhead box O3 (FOXO3a) phosphorylation in primary CD4⁺ T cells.

Toxicity: Standard Handling (A)

One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of asialo-GM₁ per min at 37°C, pH 6.0.

In 50 mM sodium acetate buffer, 0.1% LUBROL PX Detergent, pH 6.0.

Following initial thaw, aliquot and freeze (-20°C).

Mitsutake, S., et al. 1997. Anal. Biochem. 247, 52.
Sueyoshi, N., et al. 1997. J. Lipid Res. 38, 1923.
Ito, M., et al. 1995. J. Biol. Chem. 270, 24370.

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12 - Non Combustible Liquids

WGK 1