3,4-Dichloroisocoumarin-CAS 51050-59-0-Calbiochem

C₉H₄Cl₂O₂

215.03

MFCD00036960

A potent irreversible inhibitor of serine proteases. Blocks apoptotic internucleosomal DNA cleavage in thymocytes without the involvement of endonucleases. Reacts with serine proteases to release an acylchloride moiety that can acylate another active site residue. Does not affect thiol proteases and metalloproteases. Also has no activity towards β-lactamases. Effective at concentrations ranging from 5-100 µM.
Effective concentration: 5-100 µM; t_½ = 20 min at pH 7.5.
A potent, irreversible inhibitor of serine proteases. Reacts with serine proteases to release an acylchloride moiety that can acylate another active site residue. Inhibits granzyme B and blocks apoptotic internucleosomal DNA cleavage in thymocytes without the involvement of endonucleases. Does not affect thiol proteases and metalloproteases. Does not exhibit any activity towards β-lactamases.

Product does not compete with ATP.
Reversible: no
Primary Target
serine proteases
Cell permeable: no

Toxicity: Standard Handling (A)

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Cain, K., et al. 1994. Biochem. Cell Biol.72, 631.
Weaver, V.M., et al. 1993. Biochem. Cell Biol. 71, 488.
Odake, S., et al. 1991. Biochemistry 30, 2217.
Harper, J.W., et al. 1985. Biochemistry24, 1831.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

≥99% (elemental analysis)

lyophilized solid

5-100 μM IC₅₀

Calbiochem^®

OK to freeze
desiccated

white

DMF: 10 mM
DMSO: 10 mM

ambient

2-8℃

1S/C9H4Cl2O2/c10-7-5-3-1-2-4-6(5)9(12)13-8(7)11/h1-4H

SUGXUUGGLDCZKB-UHFFFAOYSA-N

10 mg in Plastic ampoule

GHS06

Danger

H301 - H315 - H319 - H335

P261 - P264 - P270 - P301 + P310 - P302 + P352 - P305 + P351 + P338

Acute Tox. 3 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Respiratory system

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK 3