Anti-Ankyrin-B Antibody, clone N105/13

32160702

NA.41

Ankyrin-2 (UniProt Q01484; also known as ANK-2, Ankyrin-B, Brain ankyrin, Brank-2, Non-erythroid ankyrin) is encoded by the ANK2 (also known as LQT4) gene (Gene ID 287) in human. Ankyrins are scaffold proteins that play essential roles in providing plasma membrane with mechanical support and in controlling excitable tissues by connecting numerous ion channels, cell adhesion molecules, and receptors to the spectrin-based cytoskeleton. There are three human ankyrins (ankyrin-R, -B, and -G encoded by ANK1, ANK2, and ANK3 gene, respectively), all share similar domain organization, including an N-terminal membrane-binding domain composed of 24 ankyrin (ANK) repeats, a spectrin-binding region comprised of two ZU5 domains, a UPA domain, a death domain (DD) and a variable C-terminal regulatory domain. The three ankyrins, however, do have distinct and non-overlapping functions in specific membrane domains coordinated by ankyrin-spectrin networks. Ankyrins mediate a wide range of physiological functions by interacting with and anchoring numerous membrane targets to specific membrane micro-domains in coordination with spectrin-based cytoskeletal structures. Examples include AnkB-mediated coordination of voltage-gated sodium channels, Na+/K+- ATPase, Na+/Ca2+ exchanger, and inositol 1,4,5-triphosphate receptors in cardiomyocytes, as well as the dystrophin/dystroglycan complex in skeletal muscles. In addition, multiple ankyrin isoforms generated through alternative splicing of a given ANK gene can further impart functional specificity and distinct subcellular distribution.

Clone N105/13 detects human, mouse, and rat Ankyrin-2/Ankyrin-B, but not Ankyrin-3/Ankyrin-G. Expected to react with spliced isoform 2 (Q01484-2), isoform 3 (Q01484-4), and isoform 4 (Q01484-5), but not isoform 5 (Q01484-7) reported by UniProt.

Epitope: Ankyrin repeats 6-14.
Recombinant protein corresponding to Ankyrin repeats 6-14 of human Ankyrin-B.

Research Category
Neuroscience
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Ankyrin-B in 10 µg of human brain tissue lysate.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Ankyrin-B in human cerebellum, human cerebral cortex, mouse cerebellum, mouse cerebral cortex, and rat brain tissue sections.
Detect Ankyrin-B using this Anti-Ankyrin-B Antibody, clone N105/13 validated for use in Western Blotting, Immunohistochemistry.
Research Sub Category
Adhesion (CAMs)

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Ankyrin-B in 10 µg of mouse brain tissue lysate.

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

Concentration: Please refer to lot specific datasheet.

100

mouse

purified immunoglobulin

primary antibodies

N105/13, monoclonal

mouse, rat, human

immunohistochemistry: suitable
western blot: suitable

IgG1κ

NP_001120965

Q01484

wet ice

human ... ANK2(287)

>200 kDa observed. 205.8 kDa (isoform 2), 433.7 kDa (isoform 3), and 204.8 kDa (isoform 4) calculated.

Stable for 1 year at 2-8°C from date of receipt.

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12 - Non Combustible Liquids

WGK 1