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Anti-Calreticulin Mouse mAb (FMC75)
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | purified antibody |
antibody product type | primary antibodies |
clone【克隆】 | FMC75, monoclonal |
form【形式】 | liquid |
does not contain【不包含】 | preservative |
species reactivity | human, monkey |
should not react with | hamster, bovine, canine, guinea pig, E. coli, mouse, rat, salmon |
manufacturer/tradename | Calbiochem® |
storage condition【储存条件】 | OK to freeze avoid repeated freeze/thaw cycles |
isotype【同位素/亚型】 | IgG1 |
shipped in【运输】 | wet ice |
storage temp.【储存温度】 | −20℃ |
packaging【包装】 | 50 μg in Plastic ampoule Please refer to vial label for lot-specific concentration. |
基本信息
General description【一般描述】 | Anti-Calreticulin, mouse monoclonal, clone FMC75, recognizes the ~63 kDa calreticulin in Molt 4, MCF-7, HeLa, K562, A431 & other cells. It is validated for FC, WB, ICC, IHC, and in enzyme immunoassay. Recognizes the ~63 kDa (apparent MW) calreticulin protein in Molt 4, MCF-7, HeLa, K562, A431, Jurkat, Hs67, Hs198, and vero cells. Protein G purified mouse monoclonal antibody. Recognizes the ~63 kDa (apparent MW) calreticulin protein. |
Immunogen【免疫原】 | a recombinant protein consisting of human calreticulin fused to maltose binding protein Human |
Application【应用】 | Enzyme Immunoassay (0.1 µg/ml) Immunoblotting (0.5 µg/ml, colorimetric; see application references) Immunocytochemistry (see application references) Immunohistochemistry (see application references) Flow Cytometry (see application references) |
Warning【警告】 | Toxicity: Standard Handling (A) |
Physical form【外形】 | In PBS, 0.1 mM PMSF, 50% glycerol, pH 7.2. |
Reconstitution【重悬】 | Following initial thaw, aliquot and freeze (-20°C). |
Analysis Note【分析说明】 | Positive Control Human Molt 4, MCF-7, HeLa, K562, A431, Jurkat, Hs67, Hs198 cells, or monkey Vero cells |
Other Notes【其他说明】 | Nauseef, W.M., et al. 1995. J. Biol. Chem. 270, 4741. Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603. Smith, M.J., and Koch, G.L.E. 1989. EMBO J.8, 3581. Does not cross-react with GrpE or Grp78. Variables associated with assay conditions will dictate the proper working dilution. |
Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
WGK | WGK 1 |