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Anti-F-actin-capping protein subunit alpha-1 Antibody
| 产品编号： | 4154362 |
| 规格： | from rabbit, purified by affinity chromatography |
| 包装规格： | 100 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-F-actin-capping protein subunit alpha-1 Antibody
capping protein (actin filament) muscle Z-line, alpha 1, CapZ alpha-1, F-actin-capping protein subunit alpha-1
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | F-actin capping protein subunit alpha (CapZ alpha) belongs to the F-actin capping protein family, which are characteristic heterodimers, consisting of an α subunit (31-36 kDa) and a β subunit (28-32 kDa), that cap the barbed end of actin filaments within all eukaryotes. Their ability to bind the actin filaments is in a manner independent of Ca2++ and requires the C-terminal end of both subunits for optimal binding. CapZ is contained within the Z-discs of striated muscle, where it functions to inhibit the polymerization and depolymerization of actin. Within vertebrates, there are three isoforms for each of CapZ’s subunits. All three alpha subunit isoforms are components of the WASH complex. Little is known about isoforms 1 and 2; however, isoform 3 is thought to be involved in spermatid morphogenesis and is exclusively observed within sperm and testis, with highest expression within the neck region of sperm. |
| Immunogen【免疫原】 | GST-tagged recombinant protein corres-ponding to human F-actin-capping protein subunit alpha-1. |
| Application【应用】 | Research Category Cell Structure Detect F-actin-capping protein subunit alpha-1 using this Anti-F-actin-capping protein subunit alpha-1 Antibody validated for use in WB & IC. Research Sub Category Cytoskeleton Western Blot (Snap i.d. Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected F-actin-capping protein subunit alpha-1 in NIH/3T3, HeLa, and A431 cells. |
| Quality【质量】 | Evaluated by Western Blot in HeLa cell lysate. Western Blot Analysis: 0.1 µg/mL of this antibody detected F-actin-capping protein subunit alpha-1 on 10 µg of HeLa cell lysate. |
| Physical form【外形】 | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Affinity purified |
| Analysis Note【分析说明】 | Control HeLa cell lysate |
| Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| purified by【纯化方式】 | affinity chromatography |
| species reactivity | human, pig, mouse, rat |
| species reactivity (predicted by homology) | bovine (based on 100% sequence homology), porcine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), primate (based on 100% sequence homology), canine (based on 100% sequence homology) |
| technique(s) | immunocytochemistry: suitable western blot: suitable |
| UniProt accession no.【UniProt登记号】 | P52907 |
| shipped in【运输】 | wet ice |
产品说明
| Target description【目标描述】 | ~35 kDa |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
