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Anti-Nanog Antibody

产品编号:4154669
规格:serum, Chemicon®
包装规格:100 μL
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4154669100 μL5420
基本信息
eCl@ss
32160702
NACRES
NA.41
General description【一般描述】
NANOG is a gene expressed in embryonic stem cells (ESCs) and is thought to be a key factor in maintaining pluripotency. NANOG thought to function in concert with other factors such as POU5F1 and SOX2 to establish ESC identity. These cells offer an important area of study because of their ability to maintain pluripotency. In other words, these cells have the ability to become virtually any cell of any of the three germ layers (endoderm, ectoderm, mesoderm).
Specificity【特异性】
Recognizes Nanog, human.
Immunogen【免疫原】
Synthetic peptide from human Nanog.
Application【应用】
This Anti-Nanog Antibody is validated for use in FC, WB for the detection of Nanog.
Western blotting: 1:1,000-1:5,000. The antibody recognizes a protein of ~35 kDa on MBL-5 cell lysate. Additional bands of ~50 and ~60 kDa may be seen depending on sample and antibody dilution used.
Flow Cytometry: Cormier et al., 2006. Tissue Engineering 12:3233-3245.

Optimal working dilutions must be determined by the end user.
Research Sub Category
Pluripotent & Early Differentiation
Research Category
Stem Cell Research
Physical form【外形】
Rabbit Serum. Liquid.
Unpurified
Analysis Note【分析说明】
Control
Whole cell lysate from mouse embryonic stem cells
Other Notes【其他说明】
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information【法律信息】
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
biological source【生物来源】
rabbit
Quality Level【质量水平】
100
antibody form【抗体形式】
serum
antibody product type
primary antibodies
clone【克隆】
polyclonal
species reactivity
human, mouse
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable
western blot: suitable
input
sample type: mouse embryonic stem cell(s)
sample type: human embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
NCBI accession no.【NCBI登记号】
NM_024865.2
UniProt accession no.【UniProt登记号】
Q9H9S0
shipped in【运输】
wet ice
产品说明
Target description【目标描述】
39 kDa (MW 34 kDa)
Storage and Stability【储存及稳定性】
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
10 - Combustible liquids
WGK
WGK 1
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