Anti-phospho-BRCA2 (Ser3291) Antibody

32160702

NA.41

BRCA2 (Breast Cancer Type 2 susceptibility protein) is a tumor suppressor protein encoded by the BRCA2 gene.
The protein encoded by this gene plays a direct role in error-free double-strand break repair and homologous recombination. BRCA2 shares similarities with the tumor suppressor gene BRCA1. Inherited mutations in BRCA2 as well as BRCA1 are associated with an increased risk of developing breast or ovarian cancer. Although the structures of the BRCA1 and BRCA2 genes are very different, some functions are similar. The proteins made by both genes are essential for repairing damaged DNA and interact with the protein produced by the RAD51 gene. Together these three proteins play a role in maintaining the stability of the human genome and prevent dangerous gene rearrangements that can lead to the development of cancer.

This antibody recognizes human BRCA2 phosphorylated at and around the internal domain surrounding Ser3291.

KLH-conjugated linear peptide corresponding to human BRCA2 phosphorylated at and around the internal domain surrounding Ser3291.
Epitope: Internal Domain surrounding Ser3291

Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected BRCA2 in MCF-7 cells.

Immunohistochemistry Analysis: 1:100 dilution from a previous lot detected BRCA2 in metastatic ductal carcinoma tissue.
Anti-phospho-BRCA2 (Ser3291) Antibody is a rabbit polyclonal antibody for detection of phospho-BRCA2 (Ser3291), also known as BRCA1/BRCA2-containing complex subunit 2 & Fanconi anemia group D1 protein 3, has been validated in ICC, IHC, DB.
Research Sub Category
Cell Cycle, DNA Replication & Repair

Oncoproteins

Evaluated by Dot Blot in modified and non modified peptides of phosphorylated and non-phosphorlyated BRCA2.

Dot Blot Analysis: 2 µg/mL of this antibody detected BRCA2 in modified and non modified peptides of phosphorylated and non-phosphorlyated BRCA2.

Affinity purified

Control
Modified and non modified peptides of phosphorylated and non-phosphorlyated BRCA2

rabbit

100

affinity purified immunoglobulin

primary antibodies

polyclonal

affinity chromatography

human, horse, rhesus macaque

rat (based on 100% sequence homology), equine (based on 100% sequence homology), rhesus monkey (based on 100% sequence homology), mouse (based on 100% sequence homology), hamster (based on 100% sequence homology), canine (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), cat (based on 100% sequence homology)

dot blot: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable

NP_000050.2

P51587

wet ice

~ 390 kDa

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1