Anti-TDO2 Antibody, clone 7A6.1

32160702

NA.41

Tryptophan 2,3-dioxygenase (EC 1.13.11.11; UniProt P48775; also known as TDO, TO, TRPO, Tryptamin 2,3-dioxygenase, Tryptophan oxygenase, Tryptophan pyrrolase, Tryptophanase) is encoded by the TDO2 (also known as TDO) gene (Gene ID 6999) in human. TDO2 is a cytosolic heme-containing enzyme that exists as a homotetramer, where the N terminal residues of each monomer form part of the substrate binding site in an adjacent monomer. Tryptophan 2,3-dioxygenase (TDO2) catalyzes the first step of the kynurenine pathway, namely the conversion of tryptophan to kynurenine (Kyn), which can also be catalyzed by indoleamine 2,3-dioxygenase 1 (IDO1) or IDO2. IDO1 is expressed in tissues throughout the body, while TDO2 is predominantly expressed in the liver. Altered tryptophan metabolism and increased secretion of tryptophan metabolites are found in solid tumors, including in breast cancer. TDO2-mediated Kyn production in triple-negative breast cancer (TNBC) cells is reported to activate aryl hydrocarbon receptor (AhR), an endogenous kynurenine receptor. Inhibiting TDO2 or AhR via pharmacologic or genetic means is reported to result in increased sensitivity to anoikis, as well as decreased proliferation, migration, and invasion of TNBC cells.

Clone 7A6.1 targets an internal epitope of human and murine TDO2 (TDO).

GST-tagged recombinant human TDO2 internal fragment.

Anti-TDO2, clone 7A6.1, Cat. No. MABN1537, is a highly specific mouse monoclonal antibody that targets TDO2, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected TDO2 in human kidney, liver, and small intestine tissue sections.

Evaluated by Western Blotting in mouse liver tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected TDO2 in 10 µg of mouse liver tissue lysate.

Protein G purified.
Format: Purified

Concentration: Please refer to lot specific datasheet.

100

mouse

purified immunoglobulin

primary antibodies

7A6.1, monoclonal

human, mouse

immunohistochemistry: suitable (paraffin)
western blot: suitable

IgG2aκ

NP_005642

P48775

wet ice

~48 kDa observed. 47.87 kDa (human) and 47.76 kDa (mouse) calculated.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1