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MISSION® Lenti microRNA Inhibitor, Human

产品编号:4160676
规格:hsa-miR-3650
包装规格:1 PKG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
41606761 PKG7540
产品别名

MISSION® Lenti microRNA Inhibitor, Human

Tough Decoy

TuD

基本信息
NACRES
NA.51
General description【一般描述】
Individual lenti microRNA inhibitors are designed using a proprietary algorithm, which is based on the work of Haraguchi, T, et al. and in collaboration with Dr. Hideo Iba, University of Tokyo. This algorithm utilizes the tough decoy (TuD) design. miRNA are known to regulate gene expression in a variety of manners, including translational repression, mRNA cleavage and deadenylation. The lentiviral microRNA Inhibitors are cloned into the TRC2-pLKO-puro vector. Co-transfection of this vector into the appropriate cell line with compatible packaging plasmids produces viral particles that can be used to transduce mammalian cells. Additionally, the Woodchuck Hepatitis Post-Transcriptional Regulatory Element2 (WPRE) is included, allowing for enhanced expression of transgenes delivered by lentiviral vectors. This lentiviral vector also carries a puromycin resistance gene for selection of cells.

  • Allows for potent inhibition of the desired miRNA
  • Lentiviral delivery format allows for efficient delivery of the inhibitor into a wide variety of cell types
  • Enables long-term inhibition without repeat transfection
Other Notes【其他说明】
Based on miRBase V19 Mature ID
Legal Information【法律信息】
MISSION is a registered trademark of sigma-aldrich Co. LLC
产品性质
Quality Level【质量水平】
200
product line【产品线】
MISSION®
concentration【浓度】
≥1x106 VP/ml (via p24 assay)
technique(s)
capture ELISA: 106 TU/mL using p24 (Volume 200 uL)
mature sequence【成熟序列】
AGGUGUGUCUGUAGAGUCC
Sanger mature/minor accession no.【Sanger mature/minor 登记号】
MIMAT0018070
Sanger microRNA accession no.【Sanger microRNA登记号】
MI0016050
shipped in【运输】
dry ice
storage temp.【储存温度】
−70℃
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 3
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