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N-Acetyl-β-D-Glucosaminidase (NAG)

产品编号:4167906
规格:sufficient for ~50 tests
包装规格:50 TESTS
产品类别:进口试剂
品牌:Roche
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
416790650 TESTS8290
产品别名

N-Acetyl-β-D-Glucosaminidase (NAG)

NAG

产品性质
usage【用途】
sufficient for ~50 tests
Quality Level【质量水平】
100
manufacturer/tradename
Roche
shipped in【运输】
ambient
storage temp.【储存温度】
room temp
packaging【包装】
1 kit containing 3 components
基本信息
General description【一般描述】
N-acetyl-β-D-glucosaminidase (NAG) is mainly used in the colorimetric assay for the determination of N-acetyl-β-D-glucosaminidase in urine. NAG hydrolyzes 3-cresolsulfonphthaleinyl-N-acetyl-D-glucosaminide to release 3-cresolsulfonphthalein, which is measured photometrically at 580nm. It is an intracellular lysosomal enzyme, present in the S3 segment of proximal tubular cells and distal nephron. Presence of NAG in the urine shows an organelle impairment in proximal tubule. Level of NAG rises in individuals over 70 years of age.
Application【应用】
N-acetyl-β-D-glucosaminidase (NAG) has been used for preparing standards for the determination of N-acetyl-β-D-glucosaminidase in urine in life-science research applications.
Preparation Note【制备说明】
Working solution: Preparation of solutions:
  1. Buffer solution
    Dissolve the contents of bottle 1 with 55 ml double-distilledwater.
  2. Substrate solution
    Dissolve the contents of bottle 2 with 55 ml solution I.
  3. Stop reagent
    Dissolve the contents of bottle 3 with 110 ml double-distilled water.

Storage conditions (working solution): Stability of solutions:
Solution II is stable for 1 month when stored at 2 to 8 ℃, protected from light.
Solution III is stable for 1 month stored at 2 to 8 ℃.

Stability of the sample:
The activity determination of the N-acetyl-β-D-glucosaminidase (NAG) should be carried out directly after collecting the sample. Turbid urines should be centrifuged and the supernatant decanted. NAG is stable for one week at 2 to 8 ℃ and for one month when stored at -15 to -25 ℃.
Other Notes【其他说明】
For life science research only. Not for use in diagnostic procedures.
Components【组分】
组份不可单独销售
Buffer Substance consisting of citric acid and potassium citrate
Lyophilized Substrate consisting of sodium 3-cresolsulfonphthaleinyl-N-acetyl-β-D-glucosaminide and borax
Stop Reagent consisting of sodium carbonate
安全信息
Pictograms【象形图】
GHS07,GHS08
Signal word【警示用语:】
Danger
Hazard Statements
H319 - H360FD
Precautionary Statements
P201 - P202 - P280 - P308 + P313 - P337 + P313 - P501
Hazard Classifications【危险分类】
Eye Irrit. 2 - Repr. 1B
Storage Class Code【储存分类代码】
6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 2
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