Plasminogen Activator Inhibitor-1, Mutant, Human, Recombinant-Calbiochem

100

≥95% (SDS-PAGE)

liquid

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles

wet ice

−70℃

50 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

Highly purified preparation of an altered form of human PAI-1 containing four mutated amino acids. Mutant PAI-1 is virtually unable to go latent and is stable at elevated temperature and pH for extended periods of time (t_½ = 145 hours at 37°C, pH 7.4). Inhibits uPA (K_i = 5.1 x 10⁶ M^-1 sec^-1) and tPA (K_i = 7.9 x 10⁵ M^-1 sec^-1).
Highly purified preparation of an altered form of human PAI-1 containing four mutated amino acids. Mutant PAI-1 is virtually unable to go latent and is stable at elevated temperature and pH for extended periods of time (t_½ = 145 h at 37°C, pH 7.4). Inhibits uPA (K_i = 5.1 x 10⁶ M^-1sec^-1) and tPA (K_i = 7.9 x 10⁵ M^-1sec^-1).

≥90% by uPA assay.
Product does not compete with ATP.
Primary Target
Plasminogen Activator
Target K_i: 5.1 x 106 M-1 sec-1, 7.9 x 105 M-1 sec-1 against uPA and tPA, respectively
Reversible: no
Cell permeable: no

Toxicity: Standard Handling (A)

In 100 mM NaCl, 50 mM sodium phosphate buffer, 1 mM EDTA, pH 6.6.

Following initial thaw, aliquot and freeze (-70°C).

Berkenpas, M.B., et al. 1995. EMBO J. 14, 2969.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

10 - Combustible liquids

WGK 1