RAD51, His·Tag^®, Pneumocystis carinii, Recombinant, E. coli

≥95% (SDS-PAGE)

100

liquid

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles

wet ice

−70℃

10 ku in Plastic ampoule
Please refer to vial label for lot-specific concentration.

Recombinant, Pneumocystis carinii RAD51 fused at the N-terminus to a His•Tag sequence and expressed in E. coli. The Pneumocystis carinii RAD51 is equivalent to bacterial RecA and the homologue of yeast RAD51. It plays a pivotal role in the homologous recombination pathway for DNA double-strand break (DSB) repair. RAD51 has been shown to colocalize with tumor suppressor proteins BRCA1 (Cat. No. 203659) and BRCA2 in DNA-damage-induced foci and RAD51 is known to interact directly with BRCA2.
Recombinant, Pneumocystis carinii RAD51 fused at the N-terminus to a His•Tag sequence and expressed in E. coli. The Pneumocystis cariniiRAD51 is equivalent to bacterial RecA and the homolog of yeast RAD51. It plays a pivotal role in the homologous recombination pathway for DNA double-strand break (DSB) repair. RAD51 has been shown to colocalize with tumor suppressor proteins BRCA1 (Cat. No. 203659) and BRCA2 in DNA-damage-induced foci and RAD51 is known to interact directly with BRCA2.

Toxicity: Standard Handling (A)

One unit equals 1 ng of purified protein. 20 units are sufficient for a DNA-protein assay and 100 units are sufficient for a protein-protein interaction assay.

In 100 mM KCl, 20 mM Tris-HCl, 1 mM DTT, 0.2 mM EDTA, 20% glycerol, pH 8.0.

West, S.C. 2003. Nat. Rev. Mol. Cell Biol.4, 435.
Jasin, M. 2002. Oncogene21, 8981.
Symington, L.S., et al. 2002. Microbiol. Mol. Biol. Rev.66, 630.
Daboussi, F., et al. 2002. Cell. Signal.14, 969.
Liu, Y. and West, S.C. 2002. Breast Cancer Res.4, 9.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

12 - Non Combustible Liquids

WGK 1