TSKgel^® Chelate-5PW HPLC Column

32110501

High efficiency, resin-based TSKgel affinity columns (10 μm particles) separate or purify many enzymes and other proteins.

TSKgel Chelate-5PW columns utilize the tendency of iminodiacetic acid (IDA) to form a complex with metal ions, such as Zn2+, Ni2+ and Cu2+. The column is loaded with divalent metal ions by chelation. Peptides and proteins containing histidine residues will normally adsorb to these chelated ions at neutral pH. The retained compounds can be eluted with buffer containing imidazole or glycine. The key to making successful use of this retention mechanism is the proper selection of metal ions for chelation and the elution buffer to desorb the analytes. In general, Cu2+, interacts better with proteins; however, resolution is usually enhanced with Zn2+. A gradient mobile phase containing increasing imidazole or glycine concentrations is used to elute the retained compounds. Alternatively, a decreasing pH gradient can be used for elution purposes as well.

Applications include: immunoglobulins, transferrin, lectins, milk proteins, membrane proteins and peptides.

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

TSKgel is a registered trademark of Tosoh Corporation

stainless steel

TSKgel^®

Tosoh Chelate-5PW, 08645

20 μmol/mL resin ligand density

≤20% organic solvent
≤3 M salt concentration
≤45 ℃ temperature
1.2 mL/min flow rate
10 bar max. pressure

HPLC: suitable

7.5 cm × 7.5 mm

polymer particle platform

iminodiacetic acid phase

10 μm

1000 Å

2 - 12

technique used for affinity HPLC

affinity