TSK-GEL^® 疏水作用 (HIC) 色谱柱

32110501

The most hydrophobic among the three TSKgel HIC columns, TSKgel Phenyl-5PW is an excellent choice to screen for the selectivity, retention and recovery of most biomolecules. Featuring a 1000Å pore size in a polymethacrylate base material, the Phenyl-5PW column is stable in either acid or caustic cleaning regimens and provides excellent access to larger molecules with low diffusion coefficients. Stainless steel is the standard column format for TSKgel Phenyl-5PW. Glass and PEEK column housings are available for samples that may adsorb to stainless steel. The alternative column formats are also suitable for general applications.
疏水作用色谱和反相液相色谱的分离都是基于蛋白质的疏水性来实现的，可以使蛋白质选择性的吸附和解吸。然而，HIC 是在极低的键合能和水性流动相下进行操作的。这些特性使得该技术更为温和，更不易干扰到蛋白质的构象。因此，HIC 通常能提供更好的活性回收率。

TSK-GEL Ether-5PW、Phenyl-5PW 和 Butyl-NPR 聚合物基质色谱柱提供了多样的疏水性选择，便于色谱性能的优化。通过使用疏水性非常强的填料可以将硫酸铵浓度降至最低(例如丁基)。推荐用 Ether-5PW 色谱柱来纯化疏水性非常强的蛋白质。Ether-5PW 和 Phenyl-5PW 填料均以 TSK-GEL G5000PW 聚合物— 孔径 1000Å 的 10μm 颗粒为基质。Butyl-NPR 填料是通过 2.5μm 无孔粒子制备的，可实现快速分析。三种色谱柱均可用 0.2M NaOH 水溶液进行冲洗。可以用相应的 Toyopearl 树脂填料来扩大规模。

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TSKgel is a registered trademark of Tosoh Corporation

PEEK

TSKgel^®

Tosoh Phenyl-5PW, 07573

≤4 M salt concentration
≤50% organic solvent
1.2 mL/min flow rate
10-50 ℃ temperature
20 bar max. pressure

HPLC: suitable

7.5 cm × 7.5 mm

polymer particle platform

Phenyl phase

10 μm

1000 Å

2 - 12

hydrophobic interaction (HIC)